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By contrast, it might be instructed that the in-vivo SSB conformations (getting entropically constrained by the greater rigid chromatin structure) can be more secure and Exhibit considerably less Recurrent spontaneous openings, as recommended from the straight-DNA results.
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considering that, the documented NMR experimental composition only offers a remaining condition with a strongly deformed hairpin, due to the interaction Together with the protein, we described also a straight DNA hairpin as Yet another initial condition for your simulations, With all the same nucleotide sequence and second contacts. through the use of this with the initial configuration, we could also stick to the entire process of SSB recognition from your earliest stage on the interaction.